In the framework of the European Synchrotron Radiation Facility (ESRF) upgrade program, a new nano-analysis beamline has been recently built on ID16 port. At 165m from the in-vacuum undulator source, ID16B endstation which offers a multimodal approach has been designed to accommodate several micro-analytical techniques (X-ray fluorescence, X-ray absorption, and X-ray diffraction) combined with 2D/3D X-ray imaging (XRI, such as magnified tomography and laminography). The beamline configuration offers an improved lateral resolution (40 nm), larger flexibility capable of in-situ experiments, and a monochromatic nanobeam tunable in a large energy range (6-65 kV). In this work, we present the different techniques available for 3D measurements at the beamline building on examples with high scientific and impact such as materials for new energy and nano-materials. We focus on ongoing developments with in-situ nano-tomography including high temperature experiments.