The degree of N-acetylation is a dominant characteristic that influences the performance of chitin in many of its applications such as drug delivery, antimicrobial activity and other aspects in tissue engineering. In this study, chitin in ?-form has been isolated from shells of crab and shrimp via chemical method, where varying concentrations of acid and alkali solutions were employed for demineralization and deproteinization processes. The contents of the different exoskeletons were analyzed and the percentage of the mineral with chitin present was determined via Thermogravimetry analysis (TGA). Degree of acetylation (DA) for the different chitin produced was conducted by the Fourier Transmission Infrared Spectroscopy (FTIR). Chitin diffraction planes, crystalline index (Crl) and crystallite size (Dhkl) were investigated by X- ray diffraction (XRD). Results showed that crab and shrimp chitin corresponded to ?- polymorph and composed of stable crystals. Chitin DA varied between 63.7- 99.4% while reflections were observed on (021), (110), (120) and (013) planes with Crl ranging between 65.6 and 87.4%.
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